Also anoxidosis environment in the zone of lesion contributes to the nonspecific anti-microbe activity of OC .
OC possesses a good biocompatibility and biodegradation, is not toxic, chemically inert, non-soluble in the
water, has a fibred structure and high mechanical durability .
A hemostatic implant on the plants basis Ankaferd Blood Stopper (ABS, Turkey) was examined for the
hemostasis from the bone tissue. An inflammatory reaction was evaluated on the amount of inflammation cells:
0-25% - weak, 25-50% - moderate, 75-100% - expressed. The factors of necrosis were considered on a qualitative
sign: presence or absence. Besides the inflammatory reaction a number of newly formed osteoblasts were
estimated. On the scale of Moretton for the implants of bone tissue Ankaferd was determined as an agent which
does not cause an active inflammation and but assisting the tissue healing . The tissue reaction caused by a
hemostatic implant is one of the reliable factors of the estimating the biocompatibility of a product and
conditioned by terms and character of biodegradation [13, 14]. Therefore, the aim of this study was to
investigate the comparative efficiency of the hemostatic implant made of the cellulose derivatives on a model of
the parenchymatous hemorrhage from a liver.
MATERIAL AND METHODS
Hemostatic Heprocel implant was worked out in SI "Republican Specialized Scientific and Practical
Medical Center of Surgery named after Academician V.Vakhidov". Basic components of implants were as follow:
sodium salt of carboxymethyl cellulose, oxidized cellulose and nanocellulose (Patent No. IAP 20160273) .
Experimental studies on the biocompatibility’s evaluation were conducted in accordance with the Russian
national standard ISO 10993-6-2011. Operations were performed under the general anaesthetizing with the
modeling the parenchymatous hemorrhage from the wound of liver. 72 white mature outbred rats of both sexes
weighing 196.5±2.8 g were used from which 36 ones made up a group of comparison with the using the
application hemostatic material of Sergicel® FibrillarTM. In the basic group of rodents (n=36) powder Heprocel
in equal amounts by weight of 30 mg was applied on a wound.
Under inhalation anesthesia (Halothanum) a supramedian laparotomy was performed. On the surface of
liver a flat wound was formed with a diameter up to one cm, and a depth up to 0.1 cm. From the liver’s wound an
active parenchymatous bleeding was marked. In the comparative group hemostasis was conducted by the
applying the hemostatic means Sergicel® FibrillarTM till the stopping the bleeding completely. In the experience
group a hemostatic powder Heprocel was applied on the bleeding surface. An observance over the possible
bleeding resumption was conducted during 10 minutes. In the fixed terms animals were taken out of an
experiment for the estimating the macroscopic changes, and also for the intake of material for histological
researches. Laboratorial animals were withdrawn from an experiment on the 1 -, 7 -, 14 -, 21 - and the 30thday
after an operation. Euthanasia was carried out according to the Provisions of ISO 10993-2-2011 under the
general anesthesia. An analysis of macroscopic picture of the abdominal cavity was made at the dissection of
animals after euthanasia.
For the making the morphological preparations ready the investigated area of liver was excised and fixed
in 10% solution of neutral for malin. After expiration of the fixing terms bioptate was inundated by paraffin in a
shape of blocks. Series of sections with a thickness of three-four µm were made. Histological preparations were
painted by hematoxylin and eosin. For the estimation of histological changes in liver a system of points was
employed in accordance with ISO 10993-6-2011 where the parameters of semi-quantitative estimation of the
number and distribution of cells characterizing the inflammatory process (polymorphonuclear neutrophils,
lymphocytes, plasmatic cells, macrophages, eosinophils and multinuclear cells) were taken into account. At the
microscopy the dynamics of the inflammatory reaction’s development, features of the liver’s parenchyma
regeneration as well as the degree of the investigated implant’s destruction were estimated.
Experimental studies had been undertaken with the observance of the rules accepted by the European
convention for the protection of vertebrate animals used for experimental and other scientific purposes (ETS N
123), Strasbourg, 18.03.1986. The obtained results were subjected to the statistical processing with the using the
standard package of Microsoft Excel 2010software by the method of variation statistics with the estimation of
indexes’ values (M±m) and distinctions of the examined selections on the Student’s t-criterion. Distinctions in
the compared groups were considered reliable at the level of value 95% (P<0.05).
The review board and ethics committee of RSCS named after acad. V.Vakhidov approved the study
protocol and informed consents were taken from all the participants. Study protocol as well as the study itself
To cite this paper: Ismailov BA, Sadykov RA, and Mirzaahmedov BM. 2019. Comparative efficacy of the hemostatic implant made of the cellulose derivatives on